Analysis of recombinant yeast decapping enzyme
نویسندگان
چکیده
منابع مشابه
Analysis of recombinant yeast decapping enzyme.
A critical step in the turnover of yeast mRNAs is decapping. Two yeast proteins, Dcp1p and Dcp2p, are absolutely required for decapping, although their precise roles in the decapping reaction have not been established. To determine the function of both Dcp1p and Dcp2p in decapping, we purified recombinant versions of these proteins from Escherichia coli and examined their properties. These expe...
متن کاملAnalysis of mutations in the yeast mRNA decapping enzyme.
A major mechanism of mRNA decay in yeast is initiated by deadenylation, followed by mRNA decapping, which exposes the transcript to 5' to 3' exonucleolytic degradation. The decapping enzyme that removes the 5' cap structure is encoded by the DCP1 gene. To understand the function of the decapping enzyme, we used alanine scanning mutagenesis to create 31 mutant versions of the enzyme, and we exam...
متن کاملIsolation and characterization of Dcp1p, the yeast mRNA decapping enzyme.
A major mechanism of mRNA decay occurs by the process of deadenylation, decapping and 5' --> 3' exonucleolytic degradation. Recently, the product of the DCP1 gene has been shown to be required for decapping mRNAs in vivo and co-purifies with decapping activity in vitro. We have purified Dcp1p to homogeneity and shown that it is sufficient for decapping, thereby indicating that Dcp1p is the deca...
متن کاملThe decapping scavenger enzyme DCS-1
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متن کاملFunctional characterization of the mammalian mRNA decapping enzyme hDcp2.
Regulation of decapping is a critical determinant of mRNA stability. We recently identified hDcp2 as a human decapping enzyme with intrinsic decapping activity. This activity is specific to N(7)-methylated guanosine containing RNA. The hDcp2 enzyme does not function on the cap structure alone and is not sensitive to competition by cap analog, suggesting that hDcp2 requires the RNA for cap recog...
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ژورنال
عنوان ژورنال: RNA
سال: 2003
ISSN: 1355-8382
DOI: 10.1261/rna.2151403